Composite

Part:BBa_K3095004

Designed by: Giulia Maria   Group: iGEM19_USP-Brazil   (2019-10-15)


pBad Ratiometric Activity Reporter

Utilizing the part BBa_K2771020 from USP-Brazil 2018 iGEM Team we constituted a part characterizing the pBad promoter and, therefore, that could be used for further uses and expressions of the pBad by just adding another part after the YFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None